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document What Is Your Quality Control (Genotyping) Strategy?
Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are carried out on our products that we report on.
15 February 2013
document Loss of Wild Type Allele (LOA)
This is a Taqman based screen in which the target primers/probe are developed within a unique wildtype region that is replaced or interrupted via homologous recombination of both vector homology arms. The data is analyzed via a multiplexed relativ...
08 March 2011
document LoxP Screen
This is the first step in the KOMP-CSD/EUCOMM production centre QC pipeline.
09 September 2010
document 5' Screen
This is the third step in the KOMP-CSD/EUCOMM production centre QC pipeline. For conditional constructs , this test (the 5’ Screen) is only performed If no long range PCR (LRPCR) bands are generated in the 3’ Screen.
09 September 2010
document 3' Screen
This is the second step in the KOMP-CSD/EUCOMM production centre QC pipeline. Long range PCR (LRPCR) is performed between a universal primer in the 3’ arm of the targeting cassette and two different genomic specific primers in the region downstrea...
09 September 2010
document Criteria for Selecting Candidates for Distribution
This matrix details the relationship between the production centre screens (LoxP Screen, 3’ Screen, 5’ Screen) and the availability of products to the distribution centres.
07 September 2010

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