International Knockout Mouse Consortium - Knowledgebase

EUCOMM Gateway protocols High Throughput Gateway (96 Well) Reactions Three Way Gateway Reaction (In tubes)

A number of criteria are applied when choosing the critical exon for a KOMP-CSD or EUCOMM allele, but not every gene can have all criteria fulfilled:

EUCOMM and KOMP-CSD Gene Targeting Cassettes Promoter-Containing Targeting Cassettes L1L2_Bact_P The targeting cassette is flanked by L1/L2 Gateway sites for insertion into R1/R2 Gateway sites upstream of the critical exon(s) in the intermediate v...

These are the EUCOMM and KOMP-CSD targeting vector plasmid backbones

Question: I am about to generate KO animals using ES cells from KOMP knockout for a particular gene using the KO first allele promoter driven approach. If I am not wrong, I can use these cells directly to generate a KO animal without any treatment...

Summary of all IKMC allele types The wild-type is represented as a transcript with three exons. The ‘floxed’ exon (the exon that will be deleted for the null allele) is exon number 2.

You can find this out yourself by following these steps:

Yes, the targeting cassette in EUCOMM and KOMP-CSD alleles, is flanked by FRT sites, such that it can be removed independently from the critical exon by means of Flip recombination. This results in the conditional allele (tm1c in the figure). Addi...

A KOMP “product” combines targeting vectors, ES cell clones, mice, and frozen germplasm generated and derived by the KOMP mutagenesis teams: the CSD consortium and Regeneron, Inc:

The KOMP Repository acquires products from the two KOMP mutagenesis teams, the CSD Consortium (CHORI, Sanger Institute, and UC Davis) and Regeneron. The following are general details of construct design for each of the two production centers.

KOMP products and services are available from the KOMP Repository. The Repository hosts the vectors, ES cells, mice, embryos, and sperm generated by the NIH KOMP Mutagenesis Project. These “products” are generated by two KOMP mutagenesis teams (th...