International Knockout Mouse Consortium - Knowledgebase

Quality control screening of IKMC ES Cells can be performed in number of different ways. This page details the different tests that are carried out on our products that we report on.

This is the first step in the KOMP-CSD/EUCOMM production centre QC pipeline.

This is the third step in the KOMP-CSD/EUCOMM production centre QC pipeline. For conditional constructs , this test (the 5’ Screen) is only performed If no long range PCR (LRPCR) bands are generated in the 3’ Screen.

This is the second step in the KOMP-CSD/EUCOMM production centre QC pipeline. Long range PCR (LRPCR) is performed between a universal primer in the 3’ arm of the targeting cassette and two different genomic specific primers in the region downstrea...

This matrix details the relationship between the production centre screens (LoxP Screen, 3’ Screen, 5’ Screen) and the availability of products to the distribution centres.