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Downloads
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EUCOMM_HighThroughputGateway96WellReactions.pdf |
| GATEWAY 96 well reactions (15-08-08) (Before attempting these protocols refer to COSHH procedure and chemical assessments) | |
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ThreeWayGWReaction.pdf |
| Three Way Gateway Reactions for Modular Gene Targeting Vector Assembly | |
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EScellsprotocol.pdf |
| Feeders for F6 Cells | |
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Protocol_for_Colony_Picking.pdf |
| Protocol for Colony Picking | |
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Preparing_DNA_for_electroporation.pdf |
| Preparing DNA for Electroporation | |
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Single_cuvette_electroporation_conditions.pdf |
| General Instructions for Single Cuvette Electroporation With DNA Targeting Vectors | |
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Medium_and_Reagents_for_JM8.N4_ES_cells.pdf |
| Medium and reagents for JM8.N4 (C57/Bl6/N) ES cells & feeders | |
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Expanding_JM8.N4_cells_for_electroporation.pdf |
| Expanding Medium and Reagents for JM8.F6 (C57/Bl6/N) ES Cells & Feeders | |
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electroporation_of_JM8.N4_cells.pdf |
| JM8.F6 Cells Electroporation Protocol | |
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Passaging_electroporated_JM8.N4_ES_Cells.pdf |
| Passaging/Splitting Cells | |
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Medium_and_Reagents_for_JM8.F6_ES_cells.pdf |
| Medium and Reagents for JM8.F6 (C57/Bl6/N) ES Cells & Feeders | |
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Expanding_JM8.F6_cells_for_electroporation.pdf |
| Expanding JM8.F6 for Electroporation | |
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Expanding_JM8_100112221922.pdf |
| JM8.F6 Cells Electroporation Protocol | |
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Passaging_electroporated_JM8.F6_ES_Cells.pdf |
| Passaging/Splitting Cells | |
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GenomicDNApurification96well_05-12-08.pdf |
| ES Cell Genomic DNA Purification in 96 Well Format | |
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ExoI_Phos_11-02-08.pdf |
| Exonuclease I/Phosphatase | |
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Genotyping_protocol_-_SequalPrep.pdf |
| 384/96 Well Long Range Polymerase Chain Reaction (LRPCR) | |
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Sequencing_reaction_setup.pdf |
| Sequencing Reaction Setup | |
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Sequencing_reaction_setup_100112222855.pdf |
| Sequencing Reaction Setup | |
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Genotypingprotocol.pdf |
| Genotyping protocol (Expand Long Template) | |
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SOP Southern (EUCOMM-ICS) June 2011.pdf |
| SOP Southern (EUCOMM-ICS) June 2011 | |
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overview_EUCOMM_mouse_generation_protocols.doc |
| For the generation of mice, several EUCOMM protocols are offered | |
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mouse_protocol_1_Sanger.pdf |
| Chimera Mouse Production by Blastocyst Injection | |
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qPCR SOP V4.2.doc |
| WTSI Neo count qPCR | |
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mouse_production_protocol__CNR.pdf |
| Mouse Production Protocol - CNR | |
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mouse_production_protocol__ICS.pdf |
| Mouse Production Protocol - ICS | |
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Conditional knockout colonies - FRT mice v2.pdf |
| Tm1c allele genotyping | |
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Gene x generic Cre mice.pdf |
| Tm1b genotyping information | |
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Genotyping by copy number by qPCR multiplex.pdf |
| Neo count qPCR multiplex | |
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tm1a allele conversion guide2.pdf |
| Allele conversion guide v2 | |