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LacZ SR-PCR

(AUG93UAG)

The LacZ short range PCR detects the presence of the LacZ reporter in the cassette. This can be used as a rapid screen on F1 chimera progeny samples to detect likely germ line transmission. The WTSI genotyping assay is shown below

Assay 5’ Forward Name 5’ Forward Sequence 3’ Reverse Name 3’ Reverse Sequence Product size
LacZ LacZ_2_small_F ATCACGACGCGCTGTATC LacZ_2_small_R ACATCGGGCAAATAATATCG 108bp

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folder WTSI: Mouse Clinic Quality Control Tests - (Mice)
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