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5' Cassette Integrity

(AUG92UAG)

The 5’ cassette check used by WTSI confirms the presence of the upstream FRT site and that the 5’ end of the cassette is still present. The assay is shown below.

Assay 5’ Forward 5’ Forward Sequence 3’ Reverse 3’ Reverse Sequence Product Size
FRT 5FRT_F AGGCGCATAACGATACCACGAT 5FRT_R CCACAACGGGTTCTTCTGTT 204bp

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folder WTSI: Mouse Clinic Quality Control Tests - (Mice)
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