Article

Which Wild-Type ES Cell Lines Have Been Used to Generate Conditional Knockout Clones?

WT ES Cell Line	Background	Remarks
E14	129P2	Used for gene trap resource only. Widely used and well established. Suitable for injections on C57BL/6 blastocysts.
JM8.parental	C57BL/6N	GLT rate determined at the WTSI for JM8.parental ES cell clones: 62%
JM8.F6	C57BL/6N
JM8.N4	C57BL/6N
JM8A1.N3	C57BL/6N	JM8.F6 derived ES cell line by removing the retroposon element in the Agouti gene by gene targeting. These cells are heterozygous for Agouti (A/a). This provides a convenient dominant coat color marker to detect germline transmission in testcrosses to C57BL/6N mice. JM8A1.N3 cells are feeder independent. With a (so far) small number the GLT rate of JM8A1.N3 clones determined: 75%

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